Description:
DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Physical Properties:
- White solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Copper-free Click reaction procedures:
- Prepare the azide-containing reagents in reaction buffer.
- Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
- Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
- Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
- Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
Nanocs Inc.是全球领先的PEG修饰剂(PEG derivatives, PEGylation试剂)供应商，拥有种类齐全的PEG衍生物，包括：NHS-PEG，马来酰亚胺PEG，巯基PEG，氨基PEG，叠氮PEG，DSPE PEG，荧光PEG等。为全世界数以百万计的客户提供产品与服务。修饰性PEG可应用于药物输送、医疗设备改造和诊断分析等领域。Nanocs提供从磷脂PEGs到荧光PEGs的多种选择，种类齐全，产品线丰富。
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