Description:
Nanocs SnapLinkBiotin LC hydrazide is a hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing molecules having oxidizable sugars or aldehydes.
Nanocs SnapLink Biotin PEG hydrazide is a pegylated biotinylation reagents for labeling glycoproteins or other carbohydrate-containing molecules. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biolinylated molecules , thus reducing aggregation of labeled substrates in solution. The PEG spacer arm also gives the biotin a long and flexible structure to minimize steric hindrance for binding to streptavidin or avdins.
These biotin hydrazide reagents enable simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.
Features:
- Glycoprotein labelingBiotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins
- Cell surface labelingBiotinylate and isolate cell surface glycoproteins
- Aldehyde-reactivereacts with aldehydes formed by periodate-oxidation of sugar groups
- Hydrazide-activatedperform reactions at pH 4 to 6 in buffers such as sodium acetate
- Irreversible forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved
Specifications:
- Appearance (color): White to off-white.
- Appearance (form): Semi-solid to solid;
- Solubility: Soluble in DMSO, DMF;
Storage Conditions:
- Store-20 0C, dessiccate. Protect from light.
Nanocs Inc.是全球领先的PEG修饰剂(PEG derivatives, PEGylation试剂)供应商，拥有种类齐全的PEG衍生物，包括：NHS-PEG，马来酰亚胺PEG，巯基PEG，氨基PEG，叠氮PEG，DSPE PEG，荧光PEG等。为全世界数以百万计的客户提供产品与服务。修饰性PEG可应用于药物输送、医疗设备改造和诊断分析等领域。Nanocs提供从磷脂PEGs到荧光PEGs的多种选择，种类齐全，产品线丰富。
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