Description:
mPEG amide acetaldehyde is a site specific Pegylation reagent with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.
Physical Properties:
- Off-white/white solid;
- Soluble in water, DMSO, DMF;
- Activity >90%; Peroxide<10ppm;>
Storage Conditions:
- Store at -20 0C
Reaction Procedures:
Generally, a 5- to 10-fold molar excess ofPEG aldehyde over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
- Pegylation buffer: Amine-free aqueousbuffer, pH5.0~6.5.
- PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
- Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. AddPEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Nanocs Inc.是全球领先的PEG修饰剂(PEG derivatives, PEGylation试剂)供应商，拥有种类齐全的PEG衍生物，包括：NHS-PEG，马来酰亚胺PEG，巯基PEG，氨基PEG，叠氮PEG，DSPE PEG，荧光PEG等。为全世界数以百万计的客户提供产品与服务。修饰性PEG可应用于药物输送、医疗设备改造和诊断分析等领域。Nanocs提供从磷脂PEGs到荧光PEGs的多种选择，种类齐全，产品线丰富。
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